Download High Performance Liquid Chromatography (HPLC) course
High Performance Liquid Chromatography (HPLC) principle video course by udemy is the Comprehensive Course to Become Professional in High Performance Liquid Chromatography (HPLC) For analytical chemistry technician.
High Performance Liquid Chromatography (HPLC) techniques are so popular and common in pharma industry, cosmetic industry, chemistry industry, food industry, polymer industry and environment laboratories.High performance liquid chromatography (HPLC) is the crucial and integral analytical tool applied in all stages of drug discovery, development, and commercialization in the pharmaceutical industry, especially. At the each step of drug development, drug substances, ingredients and drug products are controlled and monitored by using HPLC techniques.In this course, chromotography, chromatography principles, types of chromatography, instrumental design of HPLC, HPLC Analysis, Types of HPLC and Descriptors of HPLC have been discussed within all technical details.
What you’ll learn
- Understand and define basics of chromatography by learning stationary phase, mobile phase and analyte
- Explain chromatography principles by understanding of affinity theory (solubility & adsorption)
- Explain the types of chromatography based on bed type, separation mechanism, and physical states of phases
- Understand instrumental design of HPLC system by explaining HPLC compartments
- Define mission and importance of HPLC compartments such as solvent reservoir, solvent delivery system (pump), sampler unit & injector, column, dedector, monitor
- Evaluate mission of HPLC techniques in analytical chemistry by explaining qualitative and quantitative analysis
- Define types of HPLC such as Normal Phase (NP HPLC), Reversed Phase (RP HPLC), Ion Exchange (IEX) and Size Exclusion Chromatography (SEC)
- Evaluate and understand descriptors of HPLC such as Retention Time, Void Volume, Retention Factor, Selectivity, Efficiency (Teorical Plate Number), Resolution
Who this course is for:
- Anyone who is willing to learn High Performance Liquid Chromatography (HPLC)
- Anyone who wants to learn the chemistry of the High Performance Liquid Chromatography (HPLC)
- Anyone who wants to be qualified in analytical chemistry, instrumental analysis and pharmaceutical industry
- Anyone who need to use HPLC techniques in analytical chemistry and/or pharmacy in the learning process
- Anyone who desire to be master in HPLC analysis by using technical/chemical background
- Anyone who wants to get certification related with High Performance Liquid Chromatography (HPLC)
Course content
- Section 1: Introduction
- Lecture 1 Introduction
- Section 2: Introduction to High Performance Liquid Chromatography (HPLC)
- Lecture 2 Introduction to High Performance Liquid Chromatography (HPLC)
- Section 3: Chromatography
- Lecture 3 Chromatography
- Section 4: Chromatography Principles
- Lecture 4 Chromatography Principles
- Section 5: Types of Chromatography
- Lecture 5 Types of Chromatography
- Section 6: Instrumental Design of HPLC System
- Lecture 6 Simple Technical Design
- Lecture 7 Technical Design
- Lecture 8 Equipment Compartments
- Lecture 9 Solvent Reservoir
- Lecture 10 Solvent Delivery System (Pump)
- Lecture 11 Sampler Unit & Injector & Autosampler
- Lecture 12 Column
- Lecture 13 Dedector
- Lecture 14 PC Monitor
- Section 7: HPLC Analysis
- Lecture 15 HPLC in Analytical Chemistry
- Lecture 16 Qualitative Analysis
- Lecture 17 Quantitative Analysis
- Lecture 18 Quantitative Analysis Example
- Section 8: Types of HPLC
- Lecture 19 Types of HPLC
- Lecture 20 Normal Phase HPLC (NP HPLC)
- Lecture 21 Reversed Phase HPLC (RP HPLC)
- Lecture 22 Ion Exchange Chromatography (IEX)
- Lecture 23 Size Exclusion Chromatography (SEC)
- Section 9: Descriptors of HPLC & Analytical Method Development
- Lecture 24 Retention Time
- Lecture 25 Void Volume (Dead Volume)
- Lecture 26 Retention Factor
- Lecture 27 Selectivity
- Lecture 28 Efficiency (Teorical Plate Number)
- Lecture 29 Selectivity & Efficiency
- Lecture 30 Resolution
- Section 10: Conclusion
- Lecture 31 Conclusion
Course details
- Video quality: MP4 | Video: h264, 1280 × 720
- Audio quality: Audio: AAC, 44.1 KHz, 2 Ch
- Video duration: 3h 30 m
- Number of lessons: 31 lectures
- Language: English
- Compressed file size: 1.2 GB
